![96 Hour Chick Embryo Serial Section](https://kumkoniak.com/15.jpg)
![96 Hour Chick Embryo Serial Section 96 Hour Chick Embryo Serial Section](https://i5.walmartimages.com/asr/9a33ea89-03da-4db2-9425-c4cece9e966b_1.689742663825115d3f2d6cf360c414e1.jpeg)
This system involves the culturing of chick embryos from the second day to the fifth day of incubation, with associated yolk and thick and thin albumen outside the egg shell.
![96 Hour Chick Embryo Serial Section 96 Hour Chick Embryo Serial Section](https://i.pinimg.com/236x/44/04/48/440448ede56a3e01907702a5da9bde30.jpg)
We have developed a simple shell-less chick embryo culture system to study glucose-induced malformations. Conclusion: This system provides a media for in ovo experimentation for the researchers without much manipulation to the natural environment. Formation and growth of the embryonic membranes, central nervous system, circulatory system, eyes, limbs, skin, wings and folding of the body were directly observed and the developmental time line also traced in 100% embryos. Results: In this study three batches, each comprising of 6 fertilized eggs were traced for developmental mile stones via windowing technique. The developing embryos were daily taken out and day wise development was studied by just lifting the lid without sacrificing the embryo. The eggs were incubated under standard conditions. One edge of the circular window was left hinged as a lid to the shell.
96 Hour Chick Embryo Serial Section windows#
Small windows were made on the shells of fertilized chicken eggs. Islamabad Medical and dental college, Islamabad Pakistan. Objective: To device a model system for easy access to the developing embryo, maintaining a natural developing environment as close as possible. These model systems not only prove to be a realistic approach to the subject of embryology but also provide an ideal experimental medium for research. This study uses chicken embryo biological models for observing naked eye development stages. Fantasying the embryological stages falls far behind in giving an exact picture of the events to undergraduate medical students.
![96 Hour Chick Embryo Serial Section 96 Hour Chick Embryo Serial Section](https://image.slidesharecdn.com/finalchick-embryonic-development-ppt-150517111719-lva1-app6891/95/final-chick-embryonicdevelopmentppt-24-638.jpg)
Embryology teachings rely mostly on simulators. The difference between the mean values of the area of apical ectodermal ridges of experimental and control groups, as calculated by Image-J, was found to be statistically insignificant.Ĭhange in the area of the apical ectodermal ridges in experimental chicks, following phenytoin exposure, was insignificant as proven on the basis of quantification by Image-J.īackground: Human embryos are not easily available for teaching purposes.
![96 Hour Chick Embryo Serial Section 96 Hour Chick Embryo Serial Section](https://www.purposegames.com/images/games/background/490/490971.png)
The area of apical ectodermal ridges of chick wing buds was calculated by employing Image-J and subjected to statistical analysis. These sections were stained with Feulgen Nuclear and Light Green. Developing embryos were extracted 96 hours (day 4) after incubation and histological sections were cut at 5 μm thickness. The controls were injected with the same volume of normal saline. A single dose of 3.5 mg sodium phenytoin was injected into each egg of the experimental group. Sixty fertilised chicken eggs of 'Egyptian fayoumi' breed were selected and separated into experimental (B) and control (A) groups, each having 30 eggs.
96 Hour Chick Embryo Serial Section software#
To determine the effect of sodium phenytoin on the apical ectodermal ridges (AER) of chick wing buds by using the software program Image-J.ĭepartment of Anatomy, Regional Center, College of Physicians and Surgeons Pakistan (CPSP), Islamabad, from January 2014 to January 2015.
![96 Hour Chick Embryo Serial Section](https://kumkoniak.com/15.jpg)